Molecular cloning of a peroxidase gene from poplar and its expression in response to stress.
Identifieur interne : 003D70 ( Main/Exploration ); précédent : 003D69; suivant : 003D71Molecular cloning of a peroxidase gene from poplar and its expression in response to stress.
Auteurs : Eun-Kyung Bae [Corée du Sud] ; Hyoshin Lee ; Jae-Soon Lee ; Eun-Woon Noh ; Jinki JoSource :
- Tree physiology [ 0829-318X ] ; 2006.
Descripteurs français
- KwdFr :
- ARN des plantes (génétique), ARN messager (génétique), Clonage moléculaire (MeSH), Codon (génétique), Croisements génétiques (MeSH), Données de séquences moléculaires (MeSH), Myeloperoxidase (génétique), Myeloperoxidase (métabolisme), Populus (enzymologie), Populus (génétique), Protéines recombinantes (métabolisme), Régulation de l'expression des gènes codant pour des enzymes (MeSH), Régulation de l'expression des gènes végétaux (MeSH), Séquence d'acides aminés (MeSH), Séquence nucléotidique (MeSH).
- MESH :
- enzymologie : Populus.
- génétique : ARN des plantes, ARN messager, Codon, Myeloperoxidase, Populus.
- métabolisme : Myeloperoxidase, Protéines recombinantes.
- Clonage moléculaire, Croisements génétiques, Données de séquences moléculaires, Régulation de l'expression des gènes codant pour des enzymes, Régulation de l'expression des gènes végétaux, Séquence d'acides aminés, Séquence nucléotidique.
English descriptors
- KwdEn :
- Amino Acid Sequence (MeSH), Base Sequence (MeSH), Cloning, Molecular (MeSH), Codon (genetics), Crosses, Genetic (MeSH), Gene Expression Regulation, Enzymologic (MeSH), Gene Expression Regulation, Plant (MeSH), Molecular Sequence Data (MeSH), Peroxidase (genetics), Peroxidase (metabolism), Populus (enzymology), Populus (genetics), RNA, Messenger (genetics), RNA, Plant (genetics), Recombinant Proteins (metabolism).
- MESH :
- chemical , genetics : Codon, Peroxidase, RNA, Messenger, RNA, Plant.
- chemical , metabolism : Peroxidase, Recombinant Proteins.
- enzymology : Populus.
- genetics : Populus.
- Amino Acid Sequence, Base Sequence, Cloning, Molecular, Crosses, Genetic, Gene Expression Regulation, Enzymologic, Gene Expression Regulation, Plant, Molecular Sequence Data.
Abstract
To elucidate the precise functions of peroxidase in poplar (Populus alba x P. tremula var. glandulosa), we cloned a peroxidase gene (PoPOD1) from poplar suspension culture cells and examined its expression pattern in response to various stresses. PoPOD1 showed the highest homology with a bacterial-induced peroxidase gene from cotton (Gossypium hirsutum L.). The PoPOD1 gene encodes a putative 316 amino acid protein with an N-terminal signal peptide of 23 residues. The DNA blot analysis indicated that PoPOD1 is a single copy gene in the poplar genome. The RNA blot analyses indicated that PoPOD1 shows cell-culture-specific expression. Expression of PoPOD1 is down-regulated by various treatments including treatment with some metals, NaCl, methyl viologen and polyethylene glycol, and by the plant growth regulators, jasmonic acid (JA) and gibberellic acid (GA(3)). The gene is significantly up-regulated by the bacterial-elicitor laminarin and by wounding. Thus, PoPOD1 gene expression is sensitively and specifically regulated at the transcription level. Because both JA and GA3 appear to be involved in the regulation of PoPOD1 expression in poplar cells, we postulate that the peroxidase encoded by PoPOD1 plays a pivotal role in defense against pathogen invasion, possibly through the formation of a cell wall barrier over the wound.
DOI: 10.1093/treephys/26.11.1405
PubMed: 16877325
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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first="Eun-Woon" last="Noh">Eun-Woon Noh</name></author><author><name sortKey="Jo, Jinki" sort="Jo, Jinki" uniqKey="Jo J" first="Jinki" last="Jo">Jinki Jo</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2006">2006</date><idno type="RBID">pubmed:16877325</idno><idno type="pmid">16877325</idno><idno type="doi">10.1093/treephys/26.11.1405</idno><idno type="wicri:Area/Main/Corpus">003D62</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">003D62</idno><idno type="wicri:Area/Main/Curation">003D62</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">003D62</idno><idno type="wicri:Area/Main/Exploration">003D62</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Molecular cloning of a peroxidase gene from poplar and its expression in response to stress.</title><author><name sortKey="Bae, Eun Kyung" sort="Bae, Eun Kyung" uniqKey="Bae E" first="Eun-Kyung" last="Bae">Eun-Kyung Bae</name><affiliation wicri:level="1"><nlm:affiliation>Biotechnology Division, Korea Forest Research Institute, Suwon 441-350, Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Biotechnology Division, Korea Forest Research Institute, Suwon 441-350</wicri:regionArea><wicri:noRegion>Suwon 441-350</wicri:noRegion></affiliation></author><author><name sortKey="Lee, Hyoshin" sort="Lee, Hyoshin" uniqKey="Lee H" first="Hyoshin" last="Lee">Hyoshin Lee</name></author><author><name sortKey="Lee, Jae Soon" sort="Lee, Jae Soon" uniqKey="Lee J" first="Jae-Soon" last="Lee">Jae-Soon Lee</name></author><author><name sortKey="Noh, Eun Woon" sort="Noh, Eun Woon" uniqKey="Noh E" first="Eun-Woon" last="Noh">Eun-Woon Noh</name></author><author><name sortKey="Jo, Jinki" sort="Jo, Jinki" uniqKey="Jo J" first="Jinki" last="Jo">Jinki Jo</name></author></analytic><series><title level="j">Tree physiology</title><idno 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génétiques (MeSH)</term><term>Données de séquences moléculaires (MeSH)</term><term>Myeloperoxidase (génétique)</term><term>Myeloperoxidase (métabolisme)</term><term>Populus (enzymologie)</term><term>Populus (génétique)</term><term>Protéines recombinantes (métabolisme)</term><term>Régulation de l'expression des gènes codant pour des enzymes (MeSH)</term><term>Régulation de l'expression des gènes végétaux (MeSH)</term><term>Séquence d'acides aminés (MeSH)</term><term>Séquence nucléotidique (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Codon</term><term>Peroxidase</term><term>RNA, Messenger</term><term>RNA, Plant</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Peroxidase</term><term>Recombinant Proteins</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ARN des plantes</term><term>ARN messager</term><term>Codon</term><term>Myeloperoxidase</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Myeloperoxidase</term><term>Protéines recombinantes</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Base Sequence</term><term>Cloning, Molecular</term><term>Crosses, Genetic</term><term>Gene Expression Regulation, Enzymologic</term><term>Gene Expression Regulation, Plant</term><term>Molecular Sequence Data</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Clonage moléculaire</term><term>Croisements génétiques</term><term>Données de séquences moléculaires</term><term>Régulation de l'expression des gènes codant pour des 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PoPOD1 showed the highest homology with a bacterial-induced peroxidase gene from cotton (Gossypium hirsutum L.). The PoPOD1 gene encodes a putative 316 amino acid protein with an N-terminal signal peptide of 23 residues. The DNA blot analysis indicated that PoPOD1 is a single copy gene in the poplar genome. The RNA blot analyses indicated that PoPOD1 shows cell-culture-specific expression. Expression of PoPOD1 is down-regulated by various treatments including treatment with some metals, NaCl, methyl viologen and polyethylene glycol, and by the plant growth regulators, jasmonic acid (JA) and gibberellic acid (GA(3)). The gene is significantly up-regulated by the bacterial-elicitor laminarin and by wounding. Thus, PoPOD1 gene expression is sensitively and specifically regulated at the transcription level. Because both JA and GA3 appear to be involved in the regulation of PoPOD1 expression in poplar cells, we postulate that the peroxidase encoded by PoPOD1 plays a pivotal role in defense against pathogen invasion, possibly through the formation of a cell wall barrier over the wound.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">16877325</PMID><DateCompleted><Year>2007</Year><Month>04</Month><Day>04</Day></DateCompleted><DateRevised><Year>2019</Year><Month>05</Month><Day>13</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0829-318X</ISSN><JournalIssue CitedMedium="Print"><Volume>26</Volume><Issue>11</Issue><PubDate><Year>2006</Year><Month>Nov</Month></PubDate></JournalIssue><Title>Tree physiology</Title><ISOAbbreviation>Tree Physiol</ISOAbbreviation></Journal><ArticleTitle>Molecular cloning of a peroxidase gene from poplar and its expression in response to stress.</ArticleTitle><Pagination><MedlinePgn>1405-12</MedlinePgn></Pagination><Abstract><AbstractText>To elucidate the precise functions of peroxidase in poplar (Populus alba x P. tremula var. glandulosa), we cloned a peroxidase gene (PoPOD1) from poplar suspension culture cells and examined its expression pattern in response to various stresses. PoPOD1 showed the highest homology with a bacterial-induced peroxidase gene from cotton (Gossypium hirsutum L.). The PoPOD1 gene encodes a putative 316 amino acid protein with an N-terminal signal peptide of 23 residues. The DNA blot analysis indicated that PoPOD1 is a single copy gene in the poplar genome. The RNA blot analyses indicated that PoPOD1 shows cell-culture-specific expression. Expression of PoPOD1 is down-regulated by various treatments including treatment with some metals, NaCl, methyl viologen and polyethylene glycol, and by the plant growth regulators, jasmonic acid (JA) and gibberellic acid (GA(3)). The gene is significantly up-regulated by the bacterial-elicitor laminarin and by wounding. Thus, PoPOD1 gene expression is sensitively and specifically regulated at the transcription level. Because both JA and GA3 appear to be involved in the regulation of PoPOD1 expression in poplar cells, we postulate that the peroxidase encoded by PoPOD1 plays a pivotal role in defense against pathogen invasion, possibly through the formation of a cell wall barrier over the wound.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Bae</LastName><ForeName>Eun-Kyung</ForeName><Initials>EK</Initials><AffiliationInfo><Affiliation>Biotechnology Division, Korea Forest Research Institute, Suwon 441-350, Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lee</LastName><ForeName>Hyoshin</ForeName><Initials>H</Initials></Author><Author ValidYN="Y"><LastName>Lee</LastName><ForeName>Jae-Soon</ForeName><Initials>JS</Initials></Author><Author ValidYN="Y"><LastName>Noh</LastName><ForeName>Eun-Woon</ForeName><Initials>EW</Initials></Author><Author ValidYN="Y"><LastName>Jo</LastName><ForeName>Jinki</ForeName><Initials>J</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Canada</Country><MedlineTA>Tree Physiol</MedlineTA><NlmUniqueID>100955338</NlmUniqueID><ISSNLinking>0829-318X</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D003062">Codon</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012333">RNA, Messenger</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D018749">RNA, Plant</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011994">Recombinant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.11.1.7</RegistryNumber><NameOfSubstance 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Proteins</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2006</Year><Month>8</Month><Day>1</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2007</Year><Month>4</Month><Day>5</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2006</Year><Month>8</Month><Day>1</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">16877325</ArticleId><ArticleId IdType="doi">10.1093/treephys/26.11.1405</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Corée du Sud</li></country></list><tree><noCountry><name sortKey="Jo, Jinki" sort="Jo, Jinki" uniqKey="Jo J" first="Jinki" last="Jo">Jinki Jo</name><name sortKey="Lee, Hyoshin" sort="Lee, Hyoshin" uniqKey="Lee H" first="Hyoshin" last="Lee">Hyoshin Lee</name><name sortKey="Lee, Jae Soon" sort="Lee, Jae Soon" uniqKey="Lee J" first="Jae-Soon" last="Lee">Jae-Soon Lee</name><name sortKey="Noh, Eun Woon" sort="Noh, Eun Woon" uniqKey="Noh E" first="Eun-Woon" last="Noh">Eun-Woon Noh</name></noCountry><country name="Corée du Sud"><noRegion><name sortKey="Bae, Eun Kyung" sort="Bae, Eun Kyung" uniqKey="Bae E" first="Eun-Kyung" last="Bae">Eun-Kyung Bae</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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